Sophisticated Analytical Instrumentation Facility, CIL and UCIM

Laboratory: FT NMR Spectrometer (400 Mhz)

 

Sophisticated multinuclear FT NMR Spectrometer model Avance-II (Bruker) is the latest acquisition in the Centre. The instrument is equipped with a cryomagnet of field strength 9.4 T. Its ¹H frequency is 400 Mhz, while for ¹³C the frequency is 100 MHz. It is equipped with automatic sample changer.

The instrument is accompanied by TOPSPIN NMR data system and features complete spectrometer control for sophisticated multipurpose applications. Other highlights being, a multicolor displaygradient amplifier accessory, auto shimming auto tuning and homo-hetronuclear de-coupling with 0.005Hz offset result.

A sensitive 5 mm multinuclear probes like BBO and inverse probe allow to study a large number of NMR sensitive nuclei such as ¹¹B, ²⁹Si, ³¹P, ⁵⁹CO, ¹¹⁹Sn.

Following experiments can be carried out on this instrument:

1. 1D ¹H and ¹³C NMR Spectrum.
2. Homonuclear Decoupling (Proton).
3. Solvent suppression experiment.
4. Heteronuclear Gated Decoupling experiment.
5. Heteronuclear Inverse Gated Decoupling experiment
6. 2D COSY and NOESY Experiments
7. DEPT-135, DEPT-90 and DEPT-45 experiments
8. HMQC
9. HMBC

Sample Requirements

1. Samples are accepted in solid or liquid form.
2. Compounds should be highly pure and soluble in commonly available solvents (chloroform, DMSO).

For ¹H NMR sample quantity should be 5 to 10 mg and for ¹³C and other nuclei 40 to 50 mg.